Mechanism of RBBP8‐mediated homologous recombination repair in gastric cancer synthetic lethal

Background It is of great clinical significance to further explore new strategies and potential combined therapeutic targets for gastric cancer. This study aimed investigate the synthetic lethal effect RBBP8 molecular intervention with a poly ADP ribose polymerase (PARP) inhibitor in non-BRCA mutant cancer clarify mechanism by which regulates homologous recombination repair. Methods The role DNA damage repair was observed using bioinformatic analysis, western blot immunofluorescence. verified 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS)and flow cytometry apoptosis experiments. Results Among patients treated chemotherapy, prognosis high expression levels worse (homologous [HR] = 1.54, p 0.028). knockdown induced had synergistic PARP treatment on cell viability inhibition AGS (generic code human adenocarcinoma cells) (t 11.154, < 0.001) N87 6.362, cells. inhibited RAD51 activation terminal excision Conclusion involved repair, into could achieve